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Clinical Chemistry 14: 243-252, 1968;
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Clinical Chemistry, Vol 14, 243-252, Copyright © 1968 by the American Association for Clinical Chemistry

Semiautomated Assay of Normal Concentrations of Urinary Glucose by an Enzymatic Fluorometric Technic

Bengt Scherstén 1 and Gunnar Tibbling 1

1 Department of Clinical Chemistry, University Hospital of Lund, Lund, Sweden.

A semiautomated, enzymatic, fluorometric method for the determination of the low glucose concentrations normally present in urine from subjects in the fasting state is described. The method is developed from a manual, enzymatic, fluorometric technic for the determination of urinary glucose using hexokinase (HK) and glucose-6-phosphate dehydrogenase (G-6-PDH). The procedure is based on measurements of the fluorescence of reduced nicotine amide adenine dinucleotide-phosphate (NADPH2) which is formed in proportion to the glucose content of the sample. The unspecific fluorescence of urine is almost completely removed by treating the urine with a mixed ion-exchange resin prior to applying the automated procedure. The chief advantage of the method is that it may afford an opportunity for low-cost screening for detection of bacteriuria.

Submitted on October 12, 1967
Accepted on November 15, 1967






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Copyright © 1968 by the American Association for Clinical Chemistry.