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Clinical Chemistry 14: 339-347, 1968;
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Clinical Chemistry, Vol 14, 339-347, Copyright © 1968 by the American Association for Clinical Chemistry

Determination of Organic Iodine Compounds in Serum

IV. A New Nonincineration Technic for Serum Thyroxine

Vincent J. Pileggi 1 and Gerald Kessler 1

1 Bio-Science Laboratories, 7600 Tyrone Ave., Van Nuys, Calif. 91405.

Unidentified substances in acetic acid column eluates, obtained in the determination of serum thyroxine by column chromatography with the anion-exchange resin Dowex-1, prevented the direct determination of thyroxine by interference with the iodidecatalyzed ceric-arsenite reaction. Pretreatment of the eluates with Br2 or Cl2 eliminated the interference and permitted the direct assay of thyroxine by the ceric-arsenite system without incineration or wet digestion. Pretreatment with Br2 or Cl2 also significantly enhanced the catalytic activity of thyroxine, rendering its iodine nearly equivalent to inorganic iodide in the ceric-arsenite system. This nonincineration thyroxine assay is significantly more specific than methods that employ wet or dry ashing, because many organic iodine compounds used in diagnosis and therapy are not measured. A comparison of the nonincineration technic with the alkaline dry-ash method on 110 random serums revealed a mean difference of 0.13 µg./100 ml. (X = 4.5 µg./ 100 ml.), with the nonincineration technic yielding the higher result. This difference was found to be statistically significant (p< 0.001).




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