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Clinical Chemistry 14: 348-359, 1968;
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Clinical Chemistry, Vol 14, 348-359, Copyright © 1968 by the American Association for Clinical Chemistry

Fluorometric Measurement of Fecal Bile Acids

Donald T. Forman 1, Charles Phillips 1, Walter Eiseman 1, and C. Bruce Taylor 1

1 Department of Pathology, Evanston Hospital, Evanston, Ill. 60201; and the Department of Biochemistry, Northwestern University Medical School, Chicago, Ill.

A method is described for the fluorometric determination of fecal bile acids. The method combines solvent extraction and thin-layer chromatography (TLC) isolation technics with a fluorometric method for measuring total bile acids. Recoveries of individual bile acids added to feces averaged 107% with a standard deviation of ± 5.6%. Bile acid excretion in normal human subjects on a self-selected mixed diet ranged from 196 to 460 mg. total bile acids per 24 hr.

Submitted on May 25, 1967






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Copyright © 1968 by the American Association for Clinical Chemistry.