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Clinical Chemistry 14: 429-436, 1968;
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Clinical Chemistry, Vol 14, 429-436, Copyright © 1968 by the American Association for Clinical Chemistry

A New Method for the Microdetermination of Free and Total Nonprotein-bound Tryptophan in Plasma

María Esther Rio 1 and Juan Claudio Sanahuja 1

1 Department of Biochemistry, School of Pharmacy and Biochemistry, University of Buenos Aires, Buenos Aires, Argentina.

A new technic for the determination of tryptophan in plasma, based on the tryptophan-dimethylaminobenzaldehyde reaction, is described. It provides a method for the determination of as little as 20 µg. of tryptophan and also permits the determination of bound tryptophan in nonprecipitable peptides, using 10% perchloric acid as the deproteinizing agent.

Submitted on August 14, 1967
Accepted on October 3, 1967






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Copyright © 1968 by the American Association for Clinical Chemistry.