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Clinical Chemistry, Vol 14, 449-455, Copyright © 1968 by the American Association for Clinical Chemistry
1 Clinical Research Center, Emory University School of Medicine; and from the
Department of Biochemistry, Emory University, Atlanta, Ga. 30322.
Uric acid added to serum is incompletely recovered in tungstic acid filtrates of serum. Contrary to previous views, the poor recovery is not the result of the adsorption of uric acid upon the precipitated protein, nor is it the result of the presence in the filtrates of inhibitors of the color-forming reaction between the sodium carbonate and phosphotungstic acid reagents, and the uric acid. Evidence is presented which implicates adsorption on filter paper as the cause of the poor recovery of uric acid from acid filtrates. Excellent recoveries are obtained when the protein precipitated by tungstic acid is removed by centrifugation.
Accepted on October 29, 1967
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