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Clinical Chemistry 14: 456-462, 1968;
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Clinical Chemistry, Vol 14, 456-462, Copyright © 1968 by the American Association for Clinical Chemistry

Micromethod for Tocopherol Determination in Blood Serum

J. Fabianek 1, J. Defilippi 1, T. Rickards 1, and A. Herp 1

1 Department of Life Sciences, New York Institute of Technology, New York, N. Y. 10023; and the Department of Biochemistry, New York Medical College, New York, N. Y. 10029.

An improved microprocedure for the determination of tocopherol in blood serum is reported. Tocopherol is oxidized by ferric chloride and the pink complex of ferrous ions with 4,7-diphenyl-10,10-phenanthroline (bathophenanthroline) is determined spectrophotometrically. The test is more sensitive than colorimetric methods using 2,2',2''-tripyridine,2,2'-bipyridine (bipyridyl) or 2,4,6-tripyridyl-s-triazine. The use of phosphoric acid prevents the photochemical reduction of ferric chloride and also reduces interference of carotene to a minimum. The amount of carotene oxidized by ferric chloride in the first 5 min. is negligible in the presence of bathophenanthroline. As its further oxidation is stopped by phosphoric acid, the contribution of carotene to the ferrous-bathophenanonthroline complex is insignificant.

Accepted on October 30, 1967




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