Clinical Chemistry
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Clinical Chemistry 14: 491-512, 1968;
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Clinical Chemistry, Vol 14, 491-512, Copyright © 1968 by the American Association for Clinical Chemistry

SYMPOSIUM ON URINARY CONSTITUENTS OF LOW MOLECULAR WEIGHT Session III

Automation of the Analysis of Urinary Steroids Using Quantitative Paper Chromatography and a Small Laboratory Digital Computer

Ian E. Bush 1

1 Department of Physiology, Medical College of Virginia, Richmond, Va.

A summary of the strategy and progress in devising a general system of biochemical analysis based on the direct photometry, or "scanning" of thin-media chromatograms is presented. Recent results suggest that the method is as precise and as accurate as other types of quantitative chromatography. It is capable of reasonably easy adoption by workers previously unfamiliar with the basic technic and apparatus. It has been subjected to more rigorous quality control by blind and double-blind trials concurrent with ordinary routine work than is usual, and has passed these tests adequately. Data processing by a small, relatively inexpensive digital computer is capable of handling the data produced by the central apparatus of the method. The sensitivity of newer instruments using well-established fluorescence reactions compares very favorably with that of flame-ionization detectors in gas-liquid chromatography but not with the electron-capture detector as yet.






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Copyright © 1968 by the American Association for Clinical Chemistry.