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Clinical Chemistry 14: 529-534, 1968;
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Clinical Chemistry, Vol 14, 529-534, Copyright © 1968 by the American Association for Clinical Chemistry

Application of Thin-Layer Chromatographic Zonal 14C-Profile Scans to the Analysis of Urinary Constituents Derived from Lipids

Fred Snyder 1 and Edgar A. Cress 1

1 Medical Division, Oak Ridge Associated Universities, Oak Ridge, Tennessee, under contract with the U. S. Atomic Energy Commission, P. O. Box 117, Oak Ridge, Tenn. 37830.

Thin-layer chromatography (TLC) has been a useful tool in the analysis of numerous urinary constituents; as in most applications of adsorption TLC, it is best used in conjunction with other chromatographic processes. The application of TLC to the resolution of polar urinary products derived from lipids is shown for the metabolism of a 14C-labeled fatty acid and a 14C-labeled glyceryl thioether. An automatic system, including fraction collection of adsorbent, pipetting, data transmission, and computer calculations, was used to obtain the TLC zonal 14C profiles.






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Copyright © 1968 by the American Association for Clinical Chemistry.