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Clinical Chemistry 14: 548-554, 1968;
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Clinical Chemistry, Vol 14, 548-554, Copyright © 1968 by the American Association for Clinical Chemistry

Glucose in Serum and Cerebrospinal Fluid by Direct Application of a Glucose Oxidase Method

Arnold G. Ware 1 and Edward P. Marbach 1

1 Department of Pathology and Laboratories of the Los Angeles County General Hospital, Los Angeles, Calif. 90033; and the Departments of Biochemistry and of Medicine, University of Southern California, School of Medicine, Los Angeles, Calif.

Glucose is measured directly in 0.02 ml. of serum or cerebrospinal fluid by reaction with glucose oxidase. The hydrogen peroxide produced reacts with iodide in the presence of a catalyst to form molecular iodine. The iodine color is proportional to the glucose and is measured photometrically. The reaction is carried out directly on serum following preincubation with molecular iodine and can be completed in 15 min. The results on normal serum average 8 mg./100 ml. lower than those obtained with the AutoAnalyzer reduction method. At a glucose concentration of 150 mg./100 ml., the procedure has a coefficient of variation of 1.4%. Recovery of added glucose averaged 98%. Hemolysis, lipemia, or icterus do not interfere.

Accepted on November 13, 1967




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Copyright © 1968 by the American Association for Clinical Chemistry.