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Clinical Chemistry 14: 615-622, 1968;
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Clinical Chemistry, Vol 14, 615-622, Copyright © 1968 by the American Association for Clinical Chemistry

A New Time-Saving Method for Determining Nitrogen in Biologic Material

M. Dambacher 1, Alice Gubler 1, and H. G. Haas 1

1 Division of Endocrinology and Metabolism, Department of Medicine, University Hospital (Bürgerspital), CH-4056 Basel, Switzerland.

A new method for the determination of nitrogen in biologic material is described. It is based on an improved ashing procedure and the Berthelot reaction in which nitrogen is converted to indophenol. The concentration of this substance is determined photometrically. The proposed method yields the same results as the Kjeldahl procedure; however, it offers a higher degree of sensitivity combined with the same degree of accuracy. In addition, the determination requires only 4 hr., in comparison to 24 hr. for the Kjeldahl method.

Submitted on May 2, 1967
Accepted on November 13, 1967






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Copyright © 1968 by the American Association for Clinical Chemistry.