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Clinical Chemistry 14: 630-636, 1968;
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Clinical Chemistry, Vol 14, 630-636, Copyright © 1968 by the American Association for Clinical Chemistry

Determination of Uric Acid An Automated Phosphotungstate Method Using NaOH as the Alkali

James L. Wheat 1

1 Department of Biochemistry, The Clifton Springs Hospital & Clinic, Clifton Springs, N. Y. 14432.

An automated method is described for the determination of serum uric acid using sodium hydroxide alone as the alkalinizing agent in the uric acid-phosphotungstate reaction. Turbidity is not a problem and antiturbidity reagents are not needed. At the rate of 40 samples per hour, there is good sensitivity and good correlation with a manual urea-cyanide method. Quality control shows a standard deviation of ± 0.1 mg./100 ml.

Submitted on November 17, 1967
Accepted on December 1, 1967







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Copyright © 1968 by the American Association for Clinical Chemistry.