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Clinical Chemistry 14: 646-659, 1968;
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Clinical Chemistry, Vol 14, 646-659, Copyright © 1968 by the American Association for Clinical Chemistry

Comparison Between Thiocholinesters and o-Nitrophenylbutyrate as Substrates in the Assay of Serum Cholinesterase

Gabor Szasz 1

1 Laboratory Department, City Hospital, Frankfurt, a.M., Höchst, Germany.

The technical and clinical usefulness of three substrates for serum cholinesterase were compared. The diagnostic value of acetylthiocholine was equal to that of butyrylthiocholine and superior to that of o-nitrophenylbutyrate. Technically, butyrylthiocholine is superior to the other two substrates chiefly because of its greater affinity for serum cholinesterase. A kinetic method using o-nitrophenylbutyrate is described that is capable of carrying out 60 tests per hour with good results.

Submitted on November 21, 1967
Accepted on December 14, 1967






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Copyright © 1968 by the American Association for Clinical Chemistry.