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Clinical Chemistry 14: 664-674, 1968;
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Clinical Chemistry, Vol 14, 664-674, Copyright © 1968 by the American Association for Clinical Chemistry

Removal of Inorganic Iodide in the Automated Serum Protein-Bound Iodine Determination

Jagan N. Ahuja 1, Alex Kaplan 1, and Paul Van Dreal 1

1 Department of Biochemistry, University Hospital, University of Washington, Seattle, Wash. 98105.

Values for serum protein-bound iodine (PBI) concentration obtained by the AutoAnalyzer method are too high because of the failure of the batch (Technicon) process to completely remove inorganic iodide. The Technicon method removes about 98% of the inorganic iodide; this incomplete adsorption of inorganic iodide would give falsely elevated PBI values in serums with high iodide concentrations.

A rapid, economical method for removing serum inorganic iodide to the extent of 99.8% by a single pass of serum through a small column packed with an anion-exchange resin is described. Using Dowex-1 x 8, 20-50 mesh, 2 ml. of serum is filtered in 30 sec. directly into an AutoAnalyzer sample cup. Filtration through the column eliminates all possibility of clogging the sample probe with either resin beads or serum clots. The column method requires a little less serum than the batch process.

Submitted on October 30, 1967
Accepted on January 4, 1968






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Copyright © 1968 by the American Association for Clinical Chemistry.