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Clinical Chemistry 14: 754-763, 1968;
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Clinical Chemistry, Vol 14, 754-763, Copyright © 1968 by the American Association for Clinical Chemistry

A Comparison of Reduced-NAD Preparations from Four Commercial Sources

R. B. McComb 1 and Royal J. Gay 1

1 Clinical Chemistry Laboratory, Hartford Hospital, Hartford, Conn. 06115.

The physical and chemical properties of several commercial sources of reduced NAD were examined. Properties compared included physical appearance, sodium and phosphorus content, ultraviolet light absorption at 260 and 340 nm, and relative rates of oxidation with two human LDH isoenzymes. Major differences between individual preparations were noted with respect to physical and chemical properties, as well as in the activity measurements. The commercial reduced-NAD preparations most rapidly oxidized by LDH were white, free-flowing substances with 260 nm:340 nm absorbance (A260:A340) ratios below 2.45. Detectable amounts of an LDH inhibitor were found in all preparations examined. A strong LDH inhibitor, which had the same properties as the inhibitor present in commercial reduced NAD, was isolated by column chromatography. This LDH inhibitor was found to produce the same degree of inhibition toward four LDH isoenzymes.

Submitted on December 7, 1967
Accepted on January 24, 1968




The following articles in journals at HighWire Press have cited this article:


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A. Kaplan, E. Weiss, S. Byrne, N. El-Torkey, and S. Margolis
Purified reduced nicotinamide adenine dinucleotide: responses to lactate dehydrogenase isozymes from three cell sources
Science, May 1, 1981; 212(4494): 553 - 555.
[Abstract] [PDF]


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Copyright © 1968 by the American Association for Clinical Chemistry.