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Clinical Chemistry 14: 890-897, 1968;
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Clinical Chemistry, Vol 14, 890-897, Copyright © 1968 by the American Association for Clinical Chemistry

A New Automated Determination of Xanthurenic Acid in Human Urine

A. Looyé 1, E. W. Kwarts 1, and A. Groen 1

1 Department of Internal Medicine and the Central Laboratory, University Hospital, Groningen, The Netherlands.

An automated determination for xanthurenic acid in urine was devised based upon its reaction with 2,6-dichloroquinonechlorimide. The separation of xanthurenic acid from interfering substances, such as urine chromogens and phenolic compounds other than xanthurenic acid, was performed by using Sephadex G-10. The effluents containing the xanthurenic acid were determined automatically. As a result of the high sensitivity of this procedure, concentration technics are unnecessary.

Submitted on November 28, 1967
Accepted on January 18, 1968






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Copyright © 1968 by the American Association for Clinical Chemistry.