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Clinical Chemistry, Vol 15, 24-30, Copyright © 1969 by the American Association for Clinical Chemistry
1 Medical Unit, The Princess Margaret Hospital, Christchurch, New Zealand.
In 3.8 N HCl at 100°, pregnanediol is selectively destroyed in the presence of pregnanolone, and urinary pregnanolone hydrolysis is complete within 5 min. A rapid gas chromatographic assay for pregnanolone in pregnancy urine is described. As many as 30 samples may be prepared for injection in 1.5 hr. Four women in the third trimester of normal pregnancy excreted 10-29 mg. of pregnanolone per 24 hr.
Submitted on April 30, 1968
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