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Clinical Chemistry 15: 961-978, 1969;
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Clinical Chemistry, Vol 15, 961-978, Copyright © 1969 by the American Association for Clinical Chemistry

A Fluorometric Method for Plasma Cortisol and Transcortin

E. K. Smith 1 and C. A. Muehlbaecher 1

1 Peters Laboratory, Children’s Orthopedic Hospital and Medical Center, 4800 Sand Point Way N.E., Seattle, Wash 98105, and Division of Endocrinology and Metabolism, Department of Pediatrics, University of Washington, Seattle, Wash 98105.

A fluorometric method for cortisol applicable to 0.3-1.0 ml plasma is described. Inclusion of a carbon tetrachloride fractionation step assures greater specificity without undue increase in required technical skill or assay time. Advantages of the fluorometric procedure over the Porter-Silber chromogen method for determination of cortisol-binding globulin (transcortin) by a gel filtration procedure are emphasized. Utilizing the methods described, plasma concentrations of cortisol and transcortin for 22 healthy adults were found to be 13.9 ± 3.9 (SD) µg/100 ml and 15.7 ± 1.3 (SD) µg bound/100 ml, respectively.

Submitted on November 2, 1968
Accepted on December 18, 1968






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Copyright © 1969 by the American Association for Clinical Chemistry.