Clinical Chemistry
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Clinical Chemistry 15: 997-1001, 1969;
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Clinical Chemistry, Vol 15, 997-1001, Copyright © 1969 by the American Association for Clinical Chemistry

Measurement of Bisulfite in Biologic Solutions

James W. Wilkins Jr. 1 and James N. Rivecca 1

1 Department of Internal Medicine, University of Michigan, Ann Arbor, Mich 48104.

In order to evaluate the toxicity of sodium bisulfite (NaHSO3) used in peritoneal dialysis solutions it was necessary to measure bisulfite in simple solutions and serum. A microdiffusion technic is described for quantification of bisulfite. This involves evolution of SO2 gas from bisulfite by acidification, trapping it as H2SO3, oxidation of the H2SO3 to H2SO4 by hydrogen peroxide, and titration of the hydrogen ion with dilute base. The method is easily performed and is quantitative in the range of 50-500 µg bisulfite per milliliter in simple solutions. Studies in serum show incomplete yet quantitatively reproducible recovery.

Submitted on April 5, 1968
Accepted on March 19, 1969






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Copyright © 1969 by the American Association for Clinical Chemistry.