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Clinical Chemistry 15: 1045-1055, 1969;
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Clinical Chemistry, Vol 15, 1045-1055, Copyright © 1969 by the American Association for Clinical Chemistry

A Bubble-gating Flow Cell for Continuous-flow Analysis

R. L. Habig 1, B. W. Schlein 1, L. Walters 1, and R. E. Thiers 1

1 Clinical Chemistry Laboratory, Department of Biochemistry, Duke University Medical Center, Durham, NC 27706.

A system is described which permits measurements, such as photometry, to be made on air-segmented streams, as in continuous-flow analysis. Elimination of the "debubbler" step before measurement provides two basic assets: (1) more rapid attainment of steady-state conditions (lower interaction between samples), and (2) maintenance of the integrity of the bubbled analysis stream, permitting subsequent handling and remeasurement. A device has been developed for detecting air bubbles. When placed across the light path of a photometer flow cell it inactivates the readout system when bubbles pass by. The resulting system measures the transmittance of each individual liquid segment of the analysis stream, while maintaining the integrity of the stream. Two examples of possible applications of this system are described. One is a simple total-protein determination with biuret, at three times the conventional sampling rate. The other is a continuous-flow, two-point, reaction-rate method for assay of alkaline phosphatase activity.

Submitted on April 14, 1969
Accepted on June 17, 1969






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Copyright © 1969 by the American Association for Clinical Chemistry.