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Clinical Chemistry 15: 1062-1071, 1969;
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Clinical Chemistry, Vol 15, 1062-1071, Copyright © 1969 by the American Association for Clinical Chemistry

A Semiquantitative Screening Test and Quantitative Assay for Total Fecal Fat

William R. Nelson 1 and Susan J. O’Hopp 1

1 Research and Development Section of the Laboratory Services, Malcolm Grow USAF Hospital, Andrews Air Force Base, Washington, DC 20331.

The gravimetric analysis of total fecal fat by a proposed chloroform extraction procedure has been shown to compare very closely with an accepted procedure involving a petroleum ether extraction, at all levels of fecal fat. The decrease in the density of the chloroform by the fecal fat is used as a semiquantitative measure of fat concentration. This is accomplished by noting the bouyancy of the chloroform extract in sodium bromide solutions of the appropriate density. If the rapid screening test indicates a possible fecal fat level above the acceptable maximum, an aliquot of the same extract is then assayed gravimetrically.

Submitted on November 22, 1968
Accepted on February 2, 1969




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Copyright © 1969 by the American Association for Clinical Chemistry.