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Clinical Chemistry 15: 1101-1123, 1969;
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Clinical Chemistry, Vol 15, 1101-1123, Copyright © 1969 by the American Association for Clinical Chemistry

Comparison of Improved Fluorometric Methods Used to Quantitate Plasma Catecholamines

William M. Manger 1, O. S. Steinsland 1, G. G. Nahas 1, Khalil G. Wakim 1, and S. Dufton 1

1 Department of Medicine and of Anesthesiology, College of Physicians and Surgeons, Columbia University, New York, and Section of Physiology, Mayo Clinic, Rochester, Minn.

Comparative studies reveal that the ethylenediamine and the trihydroxyindole method when employed as suggested are suitable for assessing sympatho-adrenal activation under various stressful conditions that are accompanied by release of catecholamines into the circulation. Both methods are very accurate in quantitating epinephrine or norepinephrine that has been added to plasma or whole blood, although recovery from blood is less, owing mainly to some association of catecholamines with formed elements, primarily erythrocytes. Use of sodium thiosulfate in the ethylenediamine procedure minimizes association of catecholamines with formed elements. If blood samples are cooled in ice water when collected, association of catecholamines with blood cells can be further reduced. In the trihydroxyindole method, unless tubes containing blood samples are immediately immersed in ice water, a significant amount of catecholamines will disappear from plasma, because of this association of catecholamines with cells.

Submitted on March 27, 1969
Accepted on May 7, 1969






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Copyright © 1969 by the American Association for Clinical Chemistry.