Clinical Chemistry
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Clinical Chemistry 15: 1157-1161, 1969;
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Clinical Chemistry, Vol 15, 1157-1161, Copyright © 1969 by the American Association for Clinical Chemistry

A Simplified Fluorometric Method for Determination of Plasma Methotrexate

Siba G. Chakrabarti 1 and I. A. Bernstein 1

1 Cellular Chemistry Laboratory, University of Michigan Medical Center, Ann Arbor, Mich 48104.

A method is described for the determination of plasma methotrexate at low concentrations. The method consists of a single fluorescence measurement on plasma proteinfree filtrates after oxidation of the drug with KMnO4 at controlled pH. The complete release of the drug into plasma filtrates is accomplished by heating the reaction mixture in a boiling water bath with occasional stirring.

Submitted on March 15, 1969
Accepted on June 30, 1969




The following articles in journals at HighWire Press have cited this article:


Home page
 Arch DermatolHome page
K. M. Halprin, K. Fukui, and A. Ohkawara
Blood Levels of Methotrexate and the Treatment of Psoriasis
Arch Dermatol, March 1, 1971; 103(3): 243 - 249.
[Abstract] [PDF]


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Copyright © 1969 by the American Association for Clinical Chemistry.