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Clinical Chemistry 15: 1171-1179, 1969;
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Clinical Chemistry, Vol 15, 1171-1179, Copyright © 1969 by the American Association for Clinical Chemistry

Serum Cholesterol Assay Using a Stable Liebermann-Burchard Reagent

Edward Kim 1 and Morris Goldberg 1

1 Department of Pathology, Norwalk Hospital, Norwalk, Conn 06856.

A single stable Liebermann-Burchard reagent was prepared and studied for the rapid and accurate determination of total cholesterol in serum. The preparation, stability, and developed spectral character of the proposed reagent were assessed. Reproducibility, recovery, and interference studies on pure standard and patient sera were performed using the Abell-Kendall method as reference. The influence of water, protein, bilirubin, hemoglobin, and other serum constituents was evaluated.

Submitted on February 15, 1969
Accepted on May 3, 1969




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Copyright © 1969 by the American Association for Clinical Chemistry.