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Clinical Chemistry 15: 1210-1217, 1969;
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Clinical Chemistry, Vol 15, 1210-1217, Copyright © 1969 by the American Association for Clinical Chemistry

Practical Acetate Membrane Overlay Method for Multiple Sample LDH Isoenzyme Separation and Quantitation

Daniel F. Beals 1, Enid Stedman 1, and D. Michael Kouns 1

1 Department of Pathology, St. Mary’s Memorial Hospital, Knoxville, Tenn 37917.

A rapid microtechnic utilizing cellulose acetate membranes for the electrophoretic separation of LDH isoenzymes is described. The use of cellulose acetate for both migration and reagent application represents a relatively inexpensive and reproducible method which avoids many of the inherent problems associated with agar gels. The technic can be used for the analysis of a relatively large number of samples. Normal distribution values of the LDH isoenzymes were established.

Submitted on October 11, 1968
Accepted on July 15, 1969




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Copyright © 1969 by the American Association for Clinical Chemistry.