Clinical Chemistry
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Clinical Chemistry 15: 1218-1229, 1969;
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Clinical Chemistry, Vol 15, 1218-1229, Copyright © 1969 by the American Association for Clinical Chemistry

Agar Gel Lipoprotein Electrophoresis: A Correlated Study with Ultracentrifugation

R. M. lammarino 1, M. Humphrey 1, and Paul Antolik 1

1 Department of Pathology, Clinical Chemistry Laboratory, and the Addison H. Gibson Laboratory of the University of Pittsburgh School of Medicine and Presbyterian-University Hospital, Pittsburgh, Pa 15213.

Interest in lipoprotein electrophoresis has been stimulated by a reappraisal of hyperlipoproteinemic syndromes and definition of five basic types according to Fredrickson (Types I, II, III, IV, V). The basic analytic reference for lipoprotein studies remains ultracentrifugal analysis. We have adapted an agar gel lipoprotein electrophoretic system employing a photometric scan and quantification of lipoprotein fractions based on post-electrophoresis Sudan Black B staining. This method of lipoprotein partitioning has been compared with lipoprotein analysis by ultracentrifugation. Statistically significant correlation between the methods is demonstrated.

Submitted on October 15, 1968
Accepted on March 8, 1969






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Copyright © 1969 by the American Association for Clinical Chemistry.