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Clinical Chemistry, Vol 15, 124-136, Copyright © 1969 by the American Association for Clinical Chemistry
-Glutamyl Transpeptidase
1 Laboratory Department, City Hospital, Frankfurt a.M.-Höchst, Germany.
A kinetic method is described for the determination of 
-glutamyl transpeptidase
activity in serum. Optimal conditions for the reaction were ascertained, normal values
in milliunits per milliliter at 25° established, and the reliability of the method examined. -Glutamyl-p-nitroanilide in ammediol-HCl buffer at pH 8.2 is employed as
substrate. The method requires only 0.1 ml. of serum and, using an automatic cuvet
positioner and recorder as well as an automatic diluter, more than 60 determinations
can be carried out in 1 hr. On the basis of a large number of determinations, the
method was shown to be technically excellent and clinically valuable.
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