Clinical Chemistry AACC Online Job Center
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Clinical Chemistry 15: 154-161, 1969;
This Article
Right arrow Full Text (PDF)
Right arrow Submit an electronic Letter to
the Editor about this paper
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Dyke, K. V.
Right arrow Articles by Szustkiewicz, C.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Dyke, K. V.
Right arrow Articles by Szustkiewicz, C.

Clinical Chemistry, Vol 15, 154-161, Copyright © 1969 by the American Association for Clinical Chemistry

An Automated Detection System for Most L-agr-Amino Acids

K. Van Dyke 1 and C. Szustkiewicz 1

1 West Virginia University Medical Center, Department of Pharmacology, Morgantown, W. Va. 26506.

An automated system for the determination of the L-agr form of the majority of amino acids is presented. The method is based upon oxidative deamination of the amino acid coupled with oxidation of o-dianisidine by hydrogen peroxide. This procedure can be used comparatively for the determination of a mixture of L-agr-amino acids or for the majority of separated L-agr-amino acids (especially in conjunction with column separations from urine and blood which give falsely positive identification with ninhydrin detection). The stereospecific nature of the L-agr-amino acid oxidase enables the investigator to quantitate the amount of L-agr-amino acid in the presence of the D-agr form. From an academic viewpoint, the extreme sensitivity and wide range of the detection system make it advantageous for the study of the enzyme itself. This automated method also may be employed to follow enzymatic reactions—e.g., those catalyzed by peptidases or racemases. The methodology is extremely convenient with good reagent stability and is much more sensitive than manometric technics.

Submitted on March 13, 1968
Accepted on June 24, 1968






HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1969 by the American Association for Clinical Chemistry.