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Clinical Chemistry 15: 162-169, 1969;
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Clinical Chemistry, Vol 15, 162-169, Copyright © 1969 by the American Association for Clinical Chemistry

A Colorimetric Method for the Determination of Serum Arginase Activity

Walter F. Loeb 1 and Robert A. Stuhlman 1

1 College of Veterinary Medicine, The Ohio State University, Columbus, Ohio 43210.

A detailed description of a method for the determination of serum arginase activity is presented. Arginase activity is demonstrated by the decrease in arginine concentration in a reaction mixture, as determined by a modification of the method of Sakaguchi. The reaction is linear until inhibited by the increasing concentration of ornithine. The cellular distribution of arginase is reviewed to determine its role in diagnosis and in the evaluation of biologic systems.

Submitted on April 30, 1968
Accepted on June 24, 1968






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Copyright © 1969 by the American Association for Clinical Chemistry.