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Clinical Chemistry 15: 190-196, 1969;
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Clinical Chemistry, Vol 15, 190-196, Copyright © 1969 by the American Association for Clinical Chemistry

Simultaneous Determination of Urinary Estriol, Pregnanediol, and Pregnanolone by Gas-Liquid Chromatography

Robert D. Baynton 1 and Donald J Campbell 1

1 Division of Clinical Biochemistry, Department of Clinical Laboratories, University Hospital; and the Department of Pathology, University of Alberta, Edmonton, Alberta, Canada.

A simplified method for following the urinary excretion of estriol, pregnanediol, and pregnanolone during the course of pregnancy is presented. The procedure has been used successfully in a large hospital laboratory. An important advantage is that the estrogen and progesterone metabolites are measured simultaneously, and separate extraction and gas-liquid chromatography is not required. The urine specimen is hydrolyzed enzymatically, extracted, and the steroids are converted to trimethylsilyl ethers. Temperature-programmed gas-liquid chromatography is carried out on a 2% neopentyl glycol succinate column using a hydrogen flame ionization detector.

Submitted on March 11, 1968
Accepted on June 28, 1968






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Copyright © 1969 by the American Association for Clinical Chemistry.