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Clinical Chemistry 15: 339-348, 1969;
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Clinical Chemistry, Vol 15, 339-348, Copyright © 1969 by the American Association for Clinical Chemistry

Aldolase

I. Colorimetric Determination

Patrick V. C. Pinto 1, Paul A. Van Dreal 1, and Alex Kaplan 1

1 Department of Biochemistry, University of Washington, Seattle, Wash 98105.

The Sigma and Boehringer reagent kits for measuring aldolase activity by a colorimetric method were examined critically. The original Sibley and Lehninger (1) procedure was more satisfactory than the modifications made by the kit manufacturers. Serum aldolase activity measured by the Sigma procedure and our modification of the Sibley-Lehninger method agreed within a standard deviation of ± 2 IU; values obtained with the Boehringer kit were about 50% low.

Submitted on June 5, 1968
Accepted on August 26, 1968






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Copyright © 1969 by the American Association for Clinical Chemistry.