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Clinical Chemistry 15: 361-366, 1969;
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Clinical Chemistry, Vol 15, 361-366, Copyright © 1969 by the American Association for Clinical Chemistry

Fluorometric Determination of Histidine

John A. Ambrose 1, Allan Crimm 1, Joe Burton 1, Kathleen Paullin 1, and Charlotte Ross 1

1 National Communicable Disease Center, Health Services and Mental Health Administration, US Department of Health, Education, and Welfare, Atlanta. Ga 30333.

An accurate and precise fluorometric procedure was developed for the determination of histidine based on the observation that o-phthalaldehyde forms a fluorophor with the analogous compound, histamine. The precision of the method was improved, and the fluorophor was stabilized for days by the addition of a phosphoric acid reagent.

Submitted on August 27, 1968






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Copyright © 1969 by the American Association for Clinical Chemistry.