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Clinical Chemistry 15: 390-392, 1969;
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Clinical Chemistry, Vol 15, 390-392, Copyright © 1969 by the American Association for Clinical Chemistry

A Modified Orcinol Reaction for RNA Determination

Mohammad Kamali 1 and Hamid Manhouri 1

1 Department of Human Biologics, State Razi Institute of Serum and Vaccin, P.O. Box 656, Tehran, Iran.

The Schneider orcinol technic for pentose estimation was modified by replacing ferric chloride with cupric acetate in glacial acetic acid as catalyst, to make the test more sensitive and less DNA-reactive. In addition, data were obtained showing that human plasma contains no detectable nucleic acid by the spectrophotometric determinations applied.

Submitted on June 18, 1968







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Copyright © 1969 by the American Association for Clinical Chemistry.