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Clinical Chemistry 15: 433-437, 1969;
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Clinical Chemistry, Vol 15, 433-437, Copyright © 1969 by the American Association for Clinical Chemistry

A Simple and Rapid Procedure for the Determination of agr Amino-nitrogen and Ammonia in Urine

T. W. Clarkson 1 and L. Ferraio 1

1 Department of Radiation Biology and Biophysics, University of Rochester School of Medicine and Dentistry, Rochester, NY 14620.

The ninhydrin-colorimetric procedure for the determination of urinary agr amino-nitrogen has been simplified by the use of the Conway microdiffusion unit to remove ammonia from the sample. In this way, urinary ammonia is measured, and at the same time an ammonia-free urine sample is ready for agr amino-nitrogen analysis. Values for total and free agr amino-nitrogen in normal urine samples were found to be similar to those previously reported by more complicated procedures.

Submitted on March 7, 1968
Accepted on October 11, 1968






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Copyright © 1969 by the American Association for Clinical Chemistry.