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Clinical Chemistry 15: 467-478, 1969;
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Clinical Chemistry, Vol 15, 467-478, Copyright © 1969 by the American Association for Clinical Chemistry

Automated Fluorometric Determination of Glucose-6-Phosphate Dehydrogenase (G6PD) and 6-Phosphogluconate Dehydrogenase (6PGD) Activities in Red Blood Cells

I. K. Tan 1 and T. P. Whitehead 1

1 Biochemistry Department, Queen Elizabeth Hospital, Birmingham 15, Great Britain.

An automated fluorometric microtechnic, suitable for screening purposes, has been developed for the quantitative determination of glucose-6-phosphate dehydrogenase (G6PD) and 6-phosphogluconate dehydrogenase (6PGD) levels in red blood cells. The method is rapid, simple, and precise. It uses 50 µl whole-blood (capillary) samples and is based on the measurement of natural fluorescence emitted by the NADPH generated during the enzymatic reactions.

Submitted on August 13, 1968
Accepted on October 11, 1968






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Copyright © 1969 by the American Association for Clinical Chemistry.