Clinical Chemistry
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Clinical Chemistry 15: 509-520, 1969;
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Clinical Chemistry, Vol 15, 509-520, Copyright © 1969 by the American Association for Clinical Chemistry

Specific Binding Radioassay of Serum Thyroxine

Sidney Nobel 1 and Fay Barnhart 1

1 Department of Pathology, University of Pittsburgh School of Medicine, Pittsburgh, Pa 15213.

Adaptation of the Murphy and Jachan method for serum thyroxine for routine use in a clinical chemistry laboratory is described. Details in the original paper have been amplified, and operations such as mixing, resin addition, and temperature control modified in the direction of greater precision and simplicity. A comparison study was made of the performance of the method in our laboratory with the reported performance in the laboratory of the original authors. In agreement with Murphy and coworkers and Cassidy et al, it was found that conditions could be established whereby the method was both reproducible and capable of yielding significant diagnostic information in the presence of iodinated radiographic compounds.

Accepted on October 10, 1968






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Copyright © 1969 by the American Association for Clinical Chemistry.