Clinical Chemistry 15: 669-679, 1969;
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Clinical Chemistry, Vol 15, 669-679, Copyright © 1969 by the American Association for Clinical Chemistry

Estimation of Calcium, Magnesium, and Phosphorus in Diet and Stool

H. Husdan 1 and A. Rapoport 1

1 Metabolic-Renal Unit and the Department of Biochemistry, Toronto Western Hospital, 399 Bathurst St, Toronto, Ontario, Canada; and the Departments of Medicine and Pathological Chemistry, University of Toronto.

A procedure that is simple and convenient for general use for the estimation of calcium, magnesium, and phosphorus in diet and stool is described. It includes a HNO3—HClO4 digestion in a micro-Kjeldahl flask. Calcium subsequently may be estimated by atomic absorption spectrophotometry or by the Clark-Collip procedure, magnesium by atomic absorption spectrophotometry, and phosphorus by the method of Fiske and SubbaRow. Chromium, used as a focal collection marker, does not interfere in the measurement of calcium and magnesium, whereas the measurement of phosphorus requires a preliminary ketone extraction of the chromium. The precision and accuracy of the procedure have been evaluated and found to be quite satisfactory.

Submitted on October 21, 1968
Accepted on December 29, 1968




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