Clinical Chemistry
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Clinical Chemistry 15: 699-707, 1969;
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by van Oss, C. J.
Right arrow Articles by Bronson, P. M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by van Oss, C. J.
Right arrow Articles by Bronson, P. M.

Clinical Chemistry, Vol 15, 699-707, Copyright © 1969 by the American Association for Clinical Chemistry

A Membrane for the Rapid Concentration of Dilute Protein Samples in an Ultrafilter

Carel J. van Oss 1, Cathryn R. McConnell 1, Russell K. Tompkins 1, and Paul M. Bronson 1

1 Department of Microbiology, School of Medicine, State University of New York at Buffalo, 207 Sherman Hall, Buffalo, NY 14214; and The Milwaukee Blood Center, Milwaukee, Wis.

The preparation of an anisotropic membrane which retains all proteins of a molecular weight of 25,000 and larger is described. It permits, with the help of appropriate ultrafiltration cells, the 400-fold concentration of 400 ml of a dilute protein solution within a few hours, avoiding all denaturation. Ultrafiltration with this membrane has been compared with a tannic acid precipitation method for the concentration of very dilute serum protein solutions. The ultrafiltration procedure resulted in a more representative recovery of the proteins that were present in the original undiluted sample, with quite fair yields. Some results are given on the proteins found in urine samples from normals and patients with disease.

Submitted on August 19, 1968
Accepted on November 17, 1968






HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1969 by the American Association for Clinical Chemistry.