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Clinical Chemistry 15: 727-729, 1969;
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Clinical Chemistry, Vol 15, 727-729, Copyright © 1969 by the American Association for Clinical Chemistry

A Simplified Procedure for the Assay of 5'-Nucleotidase

Sidney V. Rieder 1 and Mirta Otero 1

1 Chemistry Laboratory, Massachusetts General Hospital, Boston, Mass 02114.

5'-Nucleotidase* activity is measured by inhibiting phosphatase activity with 0.01 M Ni+2 and eliminating the requirement for protein removal before measurement of phosphate. The normal range for adults is given as 1.6-14.0 units/liter of serum, with no apparent difference between the sexes.

Submitted on November 19, 1968
Accepted on January 20, 1969






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Copyright © 1969 by the American Association for Clinical Chemistry.