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Clinical Chemistry 15: 864-869, 1969;
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Clinical Chemistry, Vol 15, 864-869, Copyright © 1969 by the American Association for Clinical Chemistry

An Automated Method for the Measurement of Plasma Hemoglobin

Herbert E. Spiegel 1 and Alice E. Tonchen 1

1 Clinical Chemistry Laboratory, Department of Clinical Pharmacology, Hoffmann-La Roche Inc., Nutley, NJ 07110.

An automated method for the determination of hemoglobin concentrations normally present in plasma is described. The method was developed from the manual technics of Crosby and Furth, and Hanks (2, 7). The procedure is based on the development of a green chromogen which results when hemoglobin reacts with benzidine and hydrogen peroxide. The results of the automated method compared well with the manual procedure. In addition, there was good precision over the concentration range of 1-5 mg/100 ml. Normals were found to range between 0.8 to 4.6 mg/100 ml with a mean of 2.4 mg/100 ml.

Submitted on August 15, 1968
Accepted on January 30, 1969






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Copyright © 1969 by the American Association for Clinical Chemistry.