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Clinical Chemistry 15: 879-883, 1969;
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Clinical Chemistry, Vol 15, 879-883, Copyright © 1969 by the American Association for Clinical Chemistry

A New Specific Assay for Urinary Creatinine

Surendra N. Sinha 1 and Elemer R. Gabrieli 1

1 Clinical Information Center, E. J. Meyer Memorial Hospital, Buffalo, NY 14215 and State University of New York at Buffalo.

A new specific assay for urinary creatinine is described. The procedure is based on the separation of creatinine from other urinary components by Sephadex gel filtration, and subsequent measurement of the compound at 235 nm against a phosphate buffer blank. No interference by other substances has been encountered. The standard deviations in a series of assays using standard solutions are less than 2%, and the recovery of added known amounts of creatinine from urine is excellent. The method has been found satisfactory for simultaneous determination of creatinine and hippuric acid in urine.

Submitted on October 8, 1968
Accepted on December 29, 1968






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Copyright © 1969 by the American Association for Clinical Chemistry.