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Clinical Chemistry 15: 884-890, 1969;
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Clinical Chemistry, Vol 15, 884-890, Copyright © 1969 by the American Association for Clinical Chemistry

An Improved Spectrophotometric Method for Determination of Total 3-Methoxy-4-Hydroxyphenylglycol in Urine

Nickie L. Nicholas 1, Harold Brown 1, and Anna M. Swander 1

1 Baylor General Clinical Research Center, Ben Taub General Hospital; the Metabolic Research Laboratory, Veterans Administration Hospital; and the Department of Medicine, Baylor University College of Medicine, Houston, Tex 77025.

A clinically useful spectrophotometric method for the determination of 3-methoxy-4-hydroxyphenylglycol (MHPG) is presented. After treatment with barium chloride, the urine is hydrolyzed with Glusulase (beta-glucuronidase and sulfatase).dagger The hydrolysate is passed through a column of Amberlite CG-4BDagger to remove any 3-methoxy-4-hydroxymandelic acid (MHMA) and the pH is adjusted to neutral. The MHPG is extracted with ethyl acetate and returned into potassium carbonate solution. Conversion of the MHPG to vanillin is accomplished with periodate and the vanillin is subsequently extracted with toluene and then potassium carbonate solution. The vanillin concentration is determined at 360 nm and 380 nm. The mean recovery of added MHPG standard was 54.9%. The mean daily excretion of MHPG in 23 samples from 10 normal subjects was 1.87 ± 0.6 mg (range 0.81-3.64). In 3 subjects with pheochromocytoma the excretion of MHPG was markedly elevated. There was an excellent correlation of the MHMA and MHPG excretion.

Submitted on November 13, 1968
Accepted on November 26, 1968






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Copyright © 1969 by the American Association for Clinical Chemistry.