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Clinical Chemistry, Vol 15, 891-901, Copyright © 1969 by the American Association for Clinical Chemistry
1 The Mary Imogene Bassett Hospital (affiliated with Columbia University), Cooperstown, NY 13326.
A simple automated method is described for determining the level of total free amino acids in the blood. The method utilizes the AutoAnalyzer, and is based on the formation of colored complexes by uniting free amino groups with 2,4,6-trinitrobenzene sulfonate (TNBS). Proteins do not interfere because the free amino acids are first separated by dialysis. Characteristics of the reaction and potential clinical applications of the procedure are discussed.
Submitted on November 21, 1968
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