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Clinical Chemistry 15: 902-907, 1969;
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Clinical Chemistry, Vol 15, 902-907, Copyright © 1969 by the American Association for Clinical Chemistry

A Rapid and Simple Thin-Layer Chromatographic Method for Amino Acids in Blood

William J. Culley 1

1 Mental Retardation Research Laboratory, Muscatatuck State Hospital, Butlerville, Ind 47223.

Simple and rapid thin-layer chromatographic methods, using heel or finger-tip blood, are described that are suitable for screening for inborn errors of amino acid metabolism. Serum is chromatographed without deproteinizing. A variation of this method utilizes blood impregnated on paper discs for chromatography. These methods are much faster, more sensitive, and provide better resolution of amino acids than paper chromatography. Because untreated serum- or blood-impregnated discs are placed directly on thin-layer chromatograms, these methods require considerably less working time than other thin-layer chromatographic methods.

Submitted on December 16, 1968
Accepted on January 10, 1969






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Copyright © 1969 by the American Association for Clinical Chemistry.