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Clinical Chemistry 15: 908-918, 1969;
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Clinical Chemistry, Vol 15, 908-918, Copyright © 1969 by the American Association for Clinical Chemistry

Laboratory Application of the Dextran-Coated-Charcoal Radioimmunoassay of Insulin

Nadine Poznanski 1 and Wojciech J. Poznanski 1

1 Metabolism Research Laboratory, Department of Metabolism, Ottawa Civic Hospital, 1053 Carling Ave, Ottawa 3, Canada.

A modification of the method for radioimmunoassay of insulin is described, which achieves satisfactory sensitivity while employing commercially available reagents and antiserums. These modifications consist mainly in lowering the initial percentage binding, reducing the amount of labeled insulin, and preincubating the unlabeled hormone with antiserum. Application of the linear isotope dilution formula, modified for our conditions, proves to be a reliable substitute for the standard curve.

Submitted on November 2, 1968
Accepted on January 20, 1969






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Copyright © 1969 by the American Association for Clinical Chemistry.