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Clinical Chemistry 16: 3-6, 1970;
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Clinical Chemistry, Vol 16, 3-6, Copyright © 1970 by the American Association for Clinical Chemistry

Chemical Basis of the Carbamidodiacetyl Micromethod for Estimation of Urea, Citrulline, and Carbamyl Derivatives

Michael P. Veniamin 1 and Catherine Vakirtzi-Lemonias 1

1 The Burnsides Research Laboratory, University of Illinois at Urbana-Champaign, Urbana, Ill. 61801, and The Nuclear Research Center "Democritus," Division of Biochemistry and Experimental Medicine, Aghia Paraskevi, Attica, Greece.

The chemical pathway of the carbamidodiacetyl colorimetric assay was investigated. The experimental variables that were studied include reaction temperature, heating time, and the ratio of the mineral acid mixture to the reactants. Evidence is presented establishing the involvement of either 7- or 8-methyl, or 7,8-dimethyltetrahydroimidazo(4,5-d)imidazole-2,5-diones as chromogens, all three being equally acceptable.

Submitted on August 7, 1969




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