|
|
||||||||
Clinical Chemistry, Vol 16, 3-6, Copyright © 1970 by the American Association for Clinical Chemistry
1 The Burnsides Research Laboratory, University of Illinois
at Urbana-Champaign, Urbana, Ill. 61801, and The Nuclear
Research Center "Democritus," Division of Biochemistry and
Experimental Medicine, Aghia Paraskevi, Attica, Greece.
The chemical pathway of the carbamidodiacetyl colorimetric assay was investigated. The experimental variables that were studied include reaction temperature, heating time, and the ratio of the mineral acid mixture to the reactants. Evidence is presented establishing the involvement of either 7- or 8-methyl, or 7,8-dimethyltetrahydroimidazo(4,5-d)imidazole-2,5-diones as chromogens, all three being equally acceptable.
Submitted on August 7, 1969
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |