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Clinical Chemistry 16: 32-38, 1970;
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Clinical Chemistry, Vol 16, 32-38, Copyright © 1970 by the American Association for Clinical Chemistry

New Chromogenic Substrate for Determination of Serum Amylase Activity

Bernard Klein 1, James A. Foreman 1, and Ronald L. Searcy 1

1 Department of Diagnostic Research, Hoffmann-La Roche, Inc., Nutley, N. J. 07110.

A rapid assay for serum amylase activity has been developed based on the use of a new chromogenic substrate—Cibachron Blue—amylose. The procedure requires 0.1 ml of serum and measures the production of soluble chromogen, the formation of which is linear with enzyme activity. Normal human serum has a mean amylase activity of 118 ± 37 (SD) mg dye/100 ml/15 min or, in international units, 78 ± 24 (SD) µg dye/min/ml. Excellent correlation was observed with a saccharometric amylase procedure.

Submitted on May 2, 1969






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Copyright © 1970 by the American Association for Clinical Chemistry.