Clinical Chemistry
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Clinical Chemistry 16: 32-38, 1970;
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Klein, B.
Right arrow Articles by Searcy, R. L.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Klein, B.
Right arrow Articles by Searcy, R. L.

Clinical Chemistry, Vol 16, 32-38, Copyright © 1970 by the American Association for Clinical Chemistry

New Chromogenic Substrate for Determination of Serum Amylase Activity

Bernard Klein 1, James A. Foreman 1, and Ronald L. Searcy 1

1 Department of Diagnostic Research, Hoffmann-La Roche, Inc., Nutley, N. J. 07110.

A rapid assay for serum amylase activity has been developed based on the use of a new chromogenic substrate—Cibachron Blue—amylose. The procedure requires 0.1 ml of serum and measures the production of soluble chromogen, the formation of which is linear with enzyme activity. Normal human serum has a mean amylase activity of 118 ± 37 (SD) mg dye/100 ml/15 min or, in international units, 78 ± 24 (SD) µg dye/min/ml. Excellent correlation was observed with a saccharometric amylase procedure.

Submitted on May 2, 1969




The following articles in journals at HighWire Press have cited this article:


Home page
 ANN INTERN MEDHome page
F. VINICOR, L. M. LEHRNER, R. C. KARN, and A. D. MERRITT
Hyperamylasemia in Diabetic Ketoacidosis: Sources and Significance
Ann Intern Med, August 1, 1979; 91(2): 200 - 204.
[Abstract] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1970 by the American Association for Clinical Chemistry.