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Clinical Chemistry 16: 922-924, 1970;
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Clinical Chemistry, Vol 16, 922-924, Copyright © 1970 by the American Association for Clinical Chemistry

Correlation between Results of a New T-3 Test and the Percentage of Free Thyroxine in Serum

Jack R. Leonards 1

1 Department of Biochemistry, School of Medicine, Case—Western Reserve University, Cleveland, Ohio 44106.

A new "T-3" test, based on the competition between serum-binding proteins and Sephadex G-25 for 125I-triiodothyronine, is presented. Results obtained with this commercially available system (TRILUTE) compare favorably with those obtained either by direct measurement of percentage of free thyroxine or by the resin sponge uptake method. The described system requires less time and less plasma (0.05 ml), and the Sephadex column is disposable. Since the results of the T-3 test with TRILUTE and those for the percentage of free thyroxine correlate well, results obtained with the TRILUTE system can validly be converted to percentage of free thyroxine.


Key Words: hyper- and hypothyroidism • normal values • resin sponge uptake method • radiolabeled triiodothyronine • thyroid function test

Submitted on February 26, 1970






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Copyright © 1970 by the American Association for Clinical Chemistry.