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Clinical Chemistry 16: 972-979, 1970;
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Clinical Chemistry, Vol 16, 972-979, Copyright © 1970 by the American Association for Clinical Chemistry

An Automated System for Kinetic Multiple-Point Determinations Exemplified by Serum Lactic Dehydrogenase Determination

Charles F. Fasce Jr. 1 and Robert Rej 1

1 Division of Laboratories and Research, New York State Department of Health, Albany, N.Y. 12201.

A fully automated procedure is presented for kinetic determinations and for use of the AutoAnalyzer in their study; serum lactic dehydrogenase has been used to illustrate such an application. The LDH-catalyzed oxidation of L-lactate by NAD in separate, unequal time-delay coils is determined by measuring the natural fluorescence of reduced coenzyme. The normal range for LDH activity by this method was 63-169 U/liter at 37°C.


Key Words: AutoAnalyzer • fluorometry • comparison with other methods • optimization • normal range

Submitted on April 11, 1970






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Copyright © 1970 by the American Association for Clinical Chemistry.