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Clinical Chemistry 16: 980-984, 1970;
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Clinical Chemistry, Vol 16, 980-984, Copyright © 1970 by the American Association for Clinical Chemistry

Direct Manual Determination of Serum Total Cholesterol with a Single Stable Reagent

D. R. Wybenga 1, V. J. Pileggi 1, P. H. Dirstine 1, and John Di Giorgio 1

1 Bio-Science Laboratories, 7600 Tyrone Ave., Van Nuys, Calif. 91405.

We describe a simple, direct, and specific manual method for quantitative determination of total cholesterol in serum. In the method, which requires no extraction of the serum, a single stable reagent and 50 µl of sample is used. Serum cholesterol concentrations determined by this method did not differ statistically from those obtained by the reference method of Abell et al. Hemoglobin, bilirubin, and ggr-globulin do not interfere unless present at markedly supranormal concentrations. Inter-run precision is about ±3% (95% confidence limits).


Key Words: ferric perchlorate—ethyl acetate • interference by bilirubin, hemoglobin, ggr-globulin • intermethod comparison




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