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Clinical Chemistry 16: 985-989, 1970;
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Clinical Chemistry, Vol 16, 985-989, Copyright © 1970 by the American Association for Clinical Chemistry

An Automated, Saccharogenic Method for Determining Serum Amylase Activity

Wendell R. O'Neal 1 and Nathan Gochman 1

1 Clinical Chemistry Service, Clinical Pathology Department, Clinical Center, National Institutes of Health, Bethesda, Md. 20014.

An automated adaptation of the Somogyi saccharogenic determination of serum amylase is described in which conventional AutoAnalyzer modules are used. Adequate sensitivity with short incubation is achieved by incorporating glucose oxidase and catalase in the substrate to destroy serum glucose during incubation. Maltose and other dialyzable oligosaccharides are measured with the alkaline copper-neocuproine reaction. A simultaneous blank run is performed to determine reducing substances other than glucose in serum. Precision studies and correlation with a manual saccharogenic method are presented. The normal range was determined from data for 49 healthy blood donors.


Key Words: amylase activity of urine and duodenal fluid • glucose oxidase • catalase • normal values • AutoAnalyzer • alkaline copper—neocuproine reaction • soluble starch substrate

Submitted on August 24, 1970






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Copyright © 1970 by the American Association for Clinical Chemistry.